Binding(includes Expression)

Price

from$79/protein

Speed

3-4 weeks

Output Data

Binding ClassificationKinetic ConstantsKinetic CurvesExpression Yield

Our Protein Binding workflow enables protein designers to easily validate protein binders by characterizing their interaction to one or several protein targets of interest. The assay is designed to be flexible in terms of the number of proteins and the number of concentrations to accommodate a wide range of applications. Run in Screening Mode, to quickly test new binders using 2 concentrations, and in Full Affinity Characterization, we perform a more detailed and precise analysis of the binding to a target using 4 or more concentrations.

Trusted by

Chai Discovery

Microsoft

Cradle

Escalante

Protein types

You can test pretty much any type of protein, from small peptides, miniproteins, natural ligands, VHHs, scFvs, or Fabs. We are expressing thousands of proteins every month and see expression success rates of over 90%. For information on how to design your proteins so that they express well, check out our guide here.

Targets

We can work with nearly any soluble protein target that is commercially available, or we can custom express proteins for you. Browse our target catalog for an overview of available targets. If you need a custom or proprietary target, we can help source and prepare the appropriate materials.

State-of-the-art biophysical characterization

We use the best label-free sensing methods for protein binding and state-of-the-art instrumentation: Gator Bio for BLI and Carterra for SPR.

Proprietary assay optimization and data processing

We've spent the last 2 years building the best binding data-generation pipeline with automated buffer optimization, non-specific binding detection, and custom fitting algorithms. You can trust us to get you the best data possible for your binding experiments.

Timeline

Day 0

Designs Submission

Digitally upload your protein designs sequences on our secure platform

Day 1-7

Experiment Preparation

We design and order the DNA constructs and source and QC your target protein(s)

Day 8-9

Protein Expression

We design the DNA and express your proteins in our cell-free system

Day 10-15

Kinetic Measurements

We run all the binding assays and measure the kinetic curves

Day 16-21

Data Review & Release

We review the data, perform additional assays if needed, and release the results to you

Day 0

Designs Submission

Digitally upload your protein designs sequences on our secure platform

Day 1-7

Experiment Preparation

We design and order the DNA constructs and source and QC your target protein(s)

Day 8-9

Protein Expression

We design the DNA and express your proteins in our cell-free system

Day 10-15

Kinetic Measurements

We run all the binding assays and measure the kinetic curves

Day 16-21

Data Review & Release

We review the data, perform additional assays if needed, and release the results to you

Deliverables

View sample data

Binding classification
"Yes / No" binding classification
Kinetic curves
Fitted kinetic curves based on raw BLI/SPR data
Kinetic constants*
Precise KD, Kon, Koff. *only available if you opt for "Full affinity characterization"
Expression yields
"Low / Medium / High" yield classification

Project Configurator

Answer a few questions to get accurate pricing and timeline estimates for your project.

Assay Resolution

Do you mainly want to know if designs bind at all, or do you need precise kinetic constants?

Basic Binding Screening

I mainly want to know if designs bind at all (Yes/No). I understand the two-concentration assay yields curves but not accurate KDs across a wide range.

Full Affinity Characterization (+20% cost)

I want precise affinity characterization with kinetic curves from a two-concentration assay. I understand the fitted KDs are not accurate across a wide affinity range.