Binding(includes Expression)
from$79/protein
3-4 weeks
Our Protein Binding workflow enables protein designers to easily validate protein binders by characterizing their interaction to one or several protein targets of interest. The assay is designed to be flexible in terms of the number of proteins and the number of concentrations to accommodate a wide range of applications. Run in Screening Mode, to quickly test new binders using 2 concentrations, and in Full Affinity Characterization, we perform a more detailed and precise analysis of the binding to a target using 4 or more concentrations.
Chai Discovery
Microsoft
Cradle
EscalanteProtein types
You can test pretty much any type of protein, from small peptides, miniproteins, natural ligands, VHHs, scFvs, or Fabs. We are expressing thousands of proteins every month and see expression success rates of over 90%. For information on how to design your proteins so that they express well, check out our guide here.
Targets
We can work with nearly any soluble protein target that is commercially available, or we can custom express proteins for you. Browse our target catalog for an overview of available targets. If you need a custom or proprietary target, we can help source and prepare the appropriate materials.
Proprietary assay optimization and data processing
We've spent the last 2 years building the best binding data-generation pipeline with automated buffer optimization, non-specific binding detection, and custom fitting algorithms. You can trust us to get you the best data possible for your binding experiments.
Timeline
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs and source and QC your target protein(s)
Protein Expression
We design the DNA and express your proteins in our cell-free system
Kinetic Measurements
We run all the binding assays and measure the kinetic curves
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs and source and QC your target protein(s)
Protein Expression
We design the DNA and express your proteins in our cell-free system
Kinetic Measurements
We run all the binding assays and measure the kinetic curves
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
View sample data- Binding classification"Yes / No" binding classification
- Kinetic curvesFitted kinetic curves based on raw BLI/SPR data
- Kinetic constants*Precise KD, Kon, Koff. *only available if you opt for "Full affinity characterization"
- Expression yields"Low / Medium / High" yield classification
Project Configurator
Answer a few questions to get accurate pricing and timeline estimates for your project.
Assay Resolution
Do you need precise kinetic constants or just rough estimates?
I only want to know if my designs bind (Yes/No). No need for KDs or kinetic constants.
I want to precisely characterize binding affinity for my designs. I need kinetic constants: KD, Kon, Koff.
Target
How should we source the target?
Pick a target from our library and we'll source it for you
Ship your own target to our lab
Number of Proteins
Select how many proteins you want to characterize
They validated their Binding with Adaptyv
MicrosoftValidating proteins generated by EvoDiff, a novel sequence-first protein design model.
Learn moreChai DiscoveryBenchmarking Chai-2, a zero-shot protein design model
Learn moreCradleOptimizing EGFR antibodies to improve affinity. Part of the Adaptyv EGFR Design Competition.
Learn moreEscalanteMulti-objective minibinders design and validation against PDL1 and IL7RA.
Learn moreFrequently Asked Questions
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