Expression
from$54/protein
2-3 weeks
Our Expression workflow enables comprehensive analysis of protein expression levels across hundres or thousands of designed protein variants. Using high-throughput cell-free expression systems, we provide detailed characterization of the synthesis yield across different expression conditions.
Chai Discovery
Microsoft
Cradle
EscalanteProtein types
You can test pretty much any type of protein, from small peptides, miniproteins, natural ligands, or antibody formats such as Fabs. For information on how to design your proteins so that they express well, check out our guide here.
Multiple expression systems
We currently offer E. coli- and Yeast-based cell-free expression systems. You can choose the system that best suits your protein or compare expression yields across both platforms.
Expression screening and yield quantification
We offer two different ways to quantify the expression yield: a quick screening mode using our split-GFP method and a more detailed yield quantification mode using Capillary Electrophoresis and a Quant-iT assay.
Run SEC on demand
Optionally, we can run SEC to further characterize the expressed proteins for monomeric or oligomeric states.
Timeline
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs.
Protein Expression
We express your proteins in our cell-free system and QC them
Yield Quantification
We quantify the expression yield across different expression conditions
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs.
Protein Expression
We express your proteins in our cell-free system and QC them
Yield Quantification
We quantify the expression yield across different expression conditions
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
View sample data- Split-GFP screeningRapid expression screening using split-GFP fluorescence readouts.
- Quantitative yield (CE/Quant-iT)Absolute yield quantification via capillary electrophoresis and Quant-iT assays.
- Expression QCQC of expressed proteins across systems and conditions.
- SEC analysis (optional)Size-exclusion chromatography to assess monomeric vs. oligomeric states.
Project Configurator
Answer a few questions to get accurate pricing and timeline estimates for your project.
Delivery Speed
Choose your preferred timeline for project completion
Regular processing timeline with standard pricing
Extended timeline with reduced cost for flexible projects
Number of Proteins
Select how many proteins you want to analyze
Frequently Asked Questions
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