Thermostability(includes Expression)
from$108/protein
2-3 weeks
Our Thermostability workflow allows you to characterize the thermostability of your protein variants and optimize their stability. Using nanoDSF and DLS, we measure at what temperature your protein designs start unfolding. We can test various buffer conditions, pH variants, salts, excipients, or ligand additions to evaluate their impact on Tm and aggregation. This data is essential for developing stable protein therapeutics, high-performing enzymes and robust proteins for diagnostic applications.
Chai Discovery
Microsoft
Cradle
EscalanteSupported protein formats
We support a wide range of protein formats for expression and thermostability analysis. Importantly, to be detected in the nanoDSF assay, proteins should contain a hydrophobic core with aromatic amino acids (such as tryptophan or tyrosine), as the assay relies on intrinsic fluorescence from these residues.
High sensitivity thermostability assay
We use nanoDSF to measure the thermostability of your protein variants across a wide temperature range.
Condition screening
Stability analysis across pH, salt, and buffer conditions.
Aggregation monitoring
DLS is used to detect aggregation of proteins and determine particle sizes.
Timeline
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs for your protein variants
Protein Expression
We express your proteins in our cell-free system, purify and QC them
Assay Measurements
We run all the thermostability assays and measure the melting curves and DLS data
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Designs Submission
Digitally upload your protein designs sequences on our secure platform
Experiment Preparation
We design and order the DNA constructs for your protein variants
Protein Expression
We express your proteins in our cell-free system, purify and QC them
Assay Measurements
We run all the thermostability assays and measure the melting curves and DLS data
Data Review & Release
We review the data, perform additional assays if needed, and release the results to you
Deliverables
View sample data- Melting curvesThermal unfolding profiles upon temperature increase.
- Tm valuesMelting temperatures computed from the melting curves.
- DLS aggregation analysisDynamic light scattering (DLS) for aggregation kinetics and particle size distribution.
Project Configurator
Answer a few questions to get accurate pricing and timeline estimates for your project.
Delivery Speed
Choose your preferred timeline for project completion
Regular processing timeline with standard pricing
Extended timeline with reduced cost for flexible projects
Number of Proteins
Select how many proteins you want to analyze
Frequently Asked Questions
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